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Alpha Innotech single-chip ccd video camera
Single Chip Ccd Video Camera, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/single-chip+ccd+video+camera/single+chip+ccd+video+camera/pmc05266609-115-11-21
Average 90 stars, based on 1 article reviews
single-chip ccd video camera - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Intracellular Lipid Droplets Contain Dynamic Pools of Sphingomyelin: ADRP Binds Phospholipids with High Affinity
Article Snippet: Proteins were quantitated by densitometric analysis after image acquisition using a single-chip CCD (charge-coupled device) video camera and a computer workstation (IS-500 system from Alpha Innotech, San Leandro, CA).

Article Title: Effect of sterol carrier protein-2 expression on sphingolipid distribution in plasma membrane lipid rafts/caveolae.
Article Snippet: Although sphingolipids are highly important signaling molecules enriched in lipid rafts/caveolae, relatively little is known regarding factors such as sphingolipid binding proteins that may regulate the distribution of sphingolipids to lipid rafts/caveolae of living cells.. Since early work demonstrated that sterol carrier protein-2 (SCP-2) enhanced glycosphingolipid transfer from membranes in vitro, the effect of SCP-2 expression on sphingolipid distribution to lipid rafts/caveolae in living cells was examined.. Using a non-detergent affinity chromatography method to isolate lipid rafts/caveolae and non-rafts from purified L-cell plasma membranes, it was shown that lipid rafts/caveolae were highly enriched in multiple sphingolipid species including ceramides, acidic glycosphingolipids (ganglioside GM1); neutral glycosphingolipids (monohexosides, dihexosides, globosides), and sphingomyelin as compared to non-raft domains.

Article Title: Overexpression of sterol carrier protein-2 differentially alters hepatic cholesterol accumulation in cholesterol-fed mice
Article Snippet: Proteins were quantified by densitometric analysis after image acquisition using a single-chip CCD video camera and a computer workstation (IS-500 system from Alpha Innotech, San Leandro, CA) (22).

Article Title: Stability of fatty acyl-coenzyme A thioester ligands of hepatocyte nuclear factor-4alpha and peroxisome proliferator-activated receptor-alpha.
Article Snippet: Although long-chain fatty acyl-coenzyme A (LCFACoA) thioesters are specific high-affinity ligands for hepatocyte nuclear factor-4α (HNF-4α) and peroxisome proliferator-activated receptor-α (PPARα), X-ray crystals of the respective purified recombinant ligand-binding domains (LBD) do not contain LCFA-CoA, but instead exhibit bound LCFA or have lost all ligands during the purification process, respectively.. As shown herein: (i) The acyl chain composition of LCFA bound to recombinant HNF-4α reflected that of the bacterial LCFA-CoA pool, rather than the bacterial LCFA pool. (ii) Bacteria used to produce the respective HNF-4α and PPARα contained nearly 100-fold less LCFA-CoA than LCFA. (iii) Under conditions used to crystallize LBD (at least 3 wk at room temperature in aqueous buffer), 16:1CoA was very unstable in buffer alone. (iv) In the presence of the respective nuclear receptor (i.e., HNF-4α and PPARα), LBD 70–75% of 16:1-CoA was degraded after 1 d at room temperature in the crystallization buffer, whereas as much as 94–97% of 16:1-CoA was degraded by 3 wk. (v) Cytoplasmic LCFA-CoA binding proteins such as acyl-CoA binding protein, sterol carrier protein-2, and liver-FA binding protein slowed the process of 16:1-CoA degradation proportional to their respective affinities for this ligand.. Taken together, these data for the first time indicated that the absence of LCFA-CoA in the crystallized HNF-4α and PPARα was due to the paucity of LCFA-CoA in bacteria as well as to the instability of LCFA-CoA in aqueous buffers and the conditions used for LBD crystallization.

Article Title: Overexpression of sterol carrier protein-2 differentially alters hepatic cholesterol accumulation in cholesterol-fed mice
Article Snippet: Proteins were quantified by densitometric analysis after image acquisition using a single-chip CCD video camera and a computer workstation (IS-500 system from Alpha Innotech, San Leandro, CA) ( 22 ).

Article Title: Impact of Dietary Phytol on Lipid Metabolism in SCP2/SCPx/L-FABP Null Mice
Article Snippet: To quantitate protein levels in the blots images were obtained using single-chip CCD video camera and a computer workstation (IS-500 system, Alpha Innotech, San Leandro, CA).

Article Title: Effect of SCP-x gene ablation on branched-chain fatty acid metabolism.
Article Snippet: Atshaves BP, McIntosh AL, Landrock D, Payne HR, Mackie JT, Maeda N, Ball J, Schroeder F, Kier AB.. Effect of SCP-x gene ablation on branched-chain fatty acid metabolism.. Am J Physiol Gastrointest Liver Physiol 292: G939–G951, 2007.

Article Title: LIVER TYPE FATTY ACID BINDING PROTEIN (L-FABP) GENE ABLATION REDUCES NUCLEAR LIGAND DISTRIBUTION AND PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR-α ACTIVITY IN CULTURED PRIMARY HEPATOCYTES 1
Article Snippet: Protein expression was quantified by densitometric analysis after image acquisition using a single-chip CCD (charge coupled device) video camera and a computer workstation (IS-500 system from Alpha Innotech, San Leandro, CA).



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